ETS & Oxidative Phosphorylation
Redox Energy Calculation:
- Calculate the efficiency of energy capture in the oxidation
of NADH by the electron transport system. KEY
Consider the relative reduction pattern of the ETS in the
presence of NADH (highly reduced) oxygen (highly oxidized) and
What would a plot of reduction vs. ETS components (e.g. NADH, FADH2, CoQH2, cyt C, etc.) look like? KEY
Calculations of P/O ratios
Calculate the P/O ratio (ATPs produced for each oxygen atom consumed) for the aerobic catabolism of glucose
in mammalian fast twitch muscle. Show all work in a table such
as we have used in class. KEY
Do this calculation again for glucose in insect flight muscle. KEY
- Why should this difference exist? (That is, why should evolution
favor this lower value of energy for insect flicht muscle?)
From the Final Exam Study Guide:
Electron Transport System
- Know localization.
- Know order of major components in ETS starting with
- Fatty acyl CoA
- How many ATP's are normally produced for each of these starting materials.
- What kinds of evidence do we have for ordering of components.
- Where is ATP production associated?
- What are:
- Non Heme Iron proteins
- Know what functional groups or cofactors are used by various conponents.
- Be able to explain and/or diagram the model we discussed for the coupling of electron transport and ox. phos. (chemiosmotic).
- Be able to calculate P/O ratios
- for any compounds oxidized via the ETS.
- for the aerobic catabolism of any compound given, or for which you know, its pathway of metabolism.
- Be able to trace the electron shuttle systems
- glycerol phosphate
- How is ATP/ADP transported across the inner mitochodrial membrane.
©R A Paselk
Last modified 20 April 2011