Kreb's TCA Cycle & CHO Metabolism Overview
Kreb's TCA Cycle
- In what way can the cycle be considered catalytic for AcCoA breakdown?
- Why is this elaborate system required to oxidize an acetyl group?
- Can any other molecules be completely oxidized by the cycle? Explain.
- Know chemistry of reactions in cycle.
- What is the "main line" sequence?
- Where is energy captured?
- as NADH?
- as FADH2?
- as GTP?
- Which reaction(s) is(are) irreversible?
- How is the cycle controlled?
- Be able to use the Kreb's Cycle and associated reactions to oxidize any of the intermediates of the cycle.
- Know reactions interconverting Pyruvate, oxaloacetate and PEP.
- Which of these reactions is regulated?
- How?
- Where do glutamate, alanine and aspartate enter the TCA Cycle? (See metabolic interactions handout)
Review aerobic pathway energy generation:
- How many ATP equivalents are generated in the conversion of glucose to CO2? KEY
- How many ATP equivalents are generated in the conversion of pyruvate to CO2? KEY
- How many ATP equivalents are generated in the conversion of lactatecytosol to CO2? KEY
- How many ATP equivalents are generated in the conversion of alpha-Ketoglutaratemitosol to CO2? KEY
- How many ATP equivalents are generated in the conversion of oxaloacetatemitosol to CO2? KEY
Carbohydrate Metabolism Overview
Give a pathway for the breakdown of glycerol to acetyCoA, naming enzyme types and energy steps. KEY
- Assuming aerobic metabolism, what is the net energy yield for this pathway in ATP units? KEY
Give pathways for the breakdown of Fructose, Mannose, and Galactose to acetylCoA, naming enzyme types and energy steps.
- Assuming aerobic metabolism, what is the net energy yield for these pathways in ATP units?
What are the main entrance/exit points of carbohydrate metabolism? KEY
©R A Paselk
Last modified 9 December 2008
