 Laboratory |
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| Chem 431 | ||
| Fall 2001 | Tentative | Office: SA470b |
| Office Hours: M, W 2; other times by appointment |
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Phone: x 5719 Home: 822-1116 |
| e-mail: rap1@axe |
Required Text: Ninfa & Ballou. Fundamentals Laboratory Approaches for Biochemistry and Biotechnology, Fitzgerald Science Press (1998)
Attendance: Attendance in the laboratory is required. You can not pass this course (lecture and lab) if you miss 3 or more labs. laboratory Since the introductory lecture at the beginning of each laboratory includes safety hazards and protocols as well as experimental procedures, you must be present for the introductory lecture. Many reagents have short lifetimes, are expensive, and must be made specifically for each laboratory experiment. Thus experiments may not be made up.
Supplies: Approved Eye-Protection (see safety regulations) and a bound Laboratory Notebook are required. Nitrile goves, Sharpie™ markers or equivalent, and a lab coat are recommended. I will provide used floppy disks for free upon request.
Lab Reports: I am still determining speciifcs for lab reports - watch this space!
| Myoglobin extraction | Nature Figure Just do a Figure as seen in the journal Nature, and modelled in the grading sheet. The data from your three plots should be represented. Due Thursday, September 13. |
| Isolation & Char. of Alkaline Phosphatase | Materials and Methods as in JBC (described in lab text) Due Tuesday, October 2. |
| Kinetics and Electrophoresis for Alk. Pase. | Results as in JBC (described in lab text) Due Tuesday, October 30. |
| Equilibrium Ligand Binding | Letter as in Nature, including figures, tables (as appropriate), references etc. Due Tuesday, November 30. |
| Nutmeg Triacylglycerols | Complete article as per Biochemistry (similar to JBC, but check out Biochemistry instructions to authors for details). Due ALONG WITH YOUR LAB NOTEBOOK, Monday of Finals week, December 17. |
| Week | Date |
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Reading** |
| 1 | 8/28 | Laboratory Safety; Check-in | N&B Chap. 1 & 13 |
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Introduction to biochemical instruments and methods. | |
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Gel Filtration Chromatography: Myoglobin Extraction from Hamburger | N&B Chap. 2 & J. Chem. Ed. (1997) 74 (4), pp 426-30 (library reserve) |
| 3-4 | 9/11-20 | Isolation & Characterization of Alkaline Phosphatase | N&B Chap 4, 6, & 7 |
| 5 | 9/25-27 | Enzyme Kinetics: KM, Vmax (Alkaline Phosphatase from Expt 7) | N&B Chap 6 & 8 |
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10/2-4 | Product Inhibition (Alkaline Phosphatase from Expt 7) | N&B Chap 6 & 8 |
| 7-8 | 10/9-18 | Gel Electrophoresis (Alkaline Phosphatase from Expt 7) | N&B Chap 5 |
| 9 | 10/22-26 | Equilibrium Ligand Binding | N&B Chap 10 & Boyer, Experiment 5 (library reserve) |
*Lectures covering techniques and theory will be intersperced as appropriate and as time allows.
** N&B = Ninfa & Ballou. Fundamentals Laboratory Approaches for Biochemistry and Biotechnology.
| Week | Date |
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Reading* |
| 10-16 | 10/29-12/12 | Isolation and characterization of triacylglycerols extracted from nutmeg. | Boyer, Chapter 7 & 8; Handouts to accompany Boyer Chap. 7 & 8. |
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Lab Final exam; Checkout |
| Laboratory |
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Last modified 18 October 2001