- Regulation of Gene Expression
- Bacterial Pheromones
- Enzyme Reaction Mechanisms
- Design of Enzyme Inhibitors
Proteomics. Proteomics is the study of the entire protein poulation of a cell (the proteome) and changes in this population that result from changes in physiological conditions such as stress or age. Currently, the primary effort in my lab is the use of proteomics techniques to study quorum sensing in gram-negative bacteria. The techniques that use to follow protein expression levels include 2-dimensional electrophoresis and isotope coded affinity tags along with mass spectrometry to identify proteins.
Bacterial communication. The cell density dependent regulation of genes (quorum sensing) is observed in many species of bacteria and has been reported for the regulation of sporulation, cell division, synthesis of exotoxins and antibiotics, conjugal transfer, and bioluminescence. The mechanism of sensing cell density involves a small diffusible molecule (autoinducer) synthesized by the bacteria that builds up in the medium until a threshold level is reached and transcription of a specific set of genes is initiated. Recently, autoinducers have been found in a large number of gram-negative bacteria, some of which are plant or animal pathogens.
Interspecies Communication. I am interested in the interrelationship between different species of organisms, especially the use of chemical signals used to communicate between species.
Detection of Environmental Pollution and Food Contamination. The application of bacterial reporter genes to detect toxic compounds in the environment and food. Some applications include the detection of heavy metal contamination of water and ß-lactam antibiotic contamination of dairy products.
"Chemical Synthesis of Bacterial Autoinducers and Autoinducer Analogs" Eberhard, A. & Schineller, J. B. (2000) Methods in Enzymology 305, 301-315.
"Lux Autoinducer Response in Escherichia coli and Vibrio fischeri," Thomas, M. D., Schineller, J. B., & Baldwin, T. O. (1997) in Bioluminescence and Chemiluminescence, Molecular Reporting with Photons , editors J. W. Hastings, L. J. Kricka and P. E. Stanley, J. Wiley & Sons, New York, NY.
"Control of cell division in Escherichia coli: Regulation of ftsQA involves both RpoS and SdiA-mediated autoinduction." Sitnikov, D., Schineller, J. B., Baldwin, T. O. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 336-341.
"Effect of MetalLigand Mutations on Phosphoryl Transfer Reactions Catalyzed by Escherichia coli Glutamine Synthetase," Abell, L. M., Schineller, J. B., Keck, P. J., & Villafranca, J. J. (1995) Biochemistry 34, 16695-16702.
"Transcriptional Regulation of Bioluminescence Genes from Vibrio fischeri," Sitnikov, D., Schineller, J. B., Baldwin, T. O. (1995) Molecular Microbiology 17, 801-812.